The structure of cyclocreatine is fairly flat (planar), which aids in passive diffusion across membranes. It has been used with success in an animal study, where mice suffered from a SLC6A8 (creatine transporter at the blood brain barrier) deficiency, which is not responsive to standard creatine supplementation.  This study failed to report increases in creatine stores in the brain, but noted a reduction of mental retardation associated with increased cyclocreatine and phosphorylated cyclocreatine storages.  As demonstrated by this animal study and previous ones, cyclocreatine is bioactive after oral ingestion   and may merely be a creatine mimetic, able to phosphorylate ADP via the creatine kinase system. 
This application note describes the extraction of benzodiazepine compounds from whole blood, prior to GC/MS analysis. This protocol also allows the simultaneous extraction of various other drugs of abuse classes: amphetamines, barbiturates, cocaine and opiates.
ISOLUTE® SLE+ columns with 1 mL sample capacity are used to extract whole blood samples following a straightforward sample dilution. No protein precipitation or other pre-treatment is required prior to sample loading. The sample preparation procedure delivers clean extracts, good recoveries and RSD values and LLOQs from 10 ng/mL (analyte dependant).
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Dr. J. L. Capelo obtained his degree in Chemistry by the University of Santiago de Compostela in 1994 and his . in Chemistry in 2002 by the Vigo University, Spain, where he was assistant to staff (grant) from 1994 to 1997 and from 1998 to 1999; and associated professor (Lecturer) from 1999 to 2002. He was honored with the University of Vigo’s award to the best Doctoral Thesis defended in 2002 in the Faculty of Chemistry. In February 2002, he moved to IST, Technical University of Lisbon, as a postdoctoral researcher. In December 2004, he became Research Fellow at REQUIMTE (), at the Chemistry Department of the New University of Lisbon, and in 2006 he was invited as senior lecturer in the same institution. Begging 2009 Dr. Capelo moved as Research Fellow to the University of Vigo, Physical Chemistry Department (Spain). Since 2012 Dr. Capelo is located as Assistant Professor at the Department of Chemistry, Faculdade de Ciências e Tecnologia, REQUIMTE - Universidade Nova de Lisboa (FCT-UNL), Moonte de Caparica, Portugal.